MBD3 polyclonal antibody
产品名称： MBD3 polyclonal antibody
英文名称： MBD3 polyclonal antibody
产品编号： PAB14055
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MBD3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to human MBD3.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In serum (0.05% sodium azide)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:100-1:500)
Western Blot (1:2 000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Cell lysate)
- Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U-937 NE, 60 ug) with MBD3 polyclonal antibody (Cat # PAB14055), diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk.
- Western Blot (Transfected lysate)
- TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U-2 OS).
Nuclear extracts were prepared and separated by electrophoresis.
An antibody directed against the TY1 tag (Lane 1) or crude serum (Lane 2) MBD3 polyclonal antibody (Cat # PAB14055) (diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3.
- Entrez GeneID:
- 53615
- Protein Accession#:
- O95983
- Gene Name:
- MBD3
- Gene Alias:
- -
- Gene Description:
- methyl-CpG binding domain protein 3
- Omim ID:
- 603573
- Gene Ontology:
- Hyperlink
- Gene Summary:
- DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
- Other Designations:
- -