Cdkn2a monoclonal antibody, clone 12-A1-1
产品名称： Cdkn2a monoclonal antibody, clone 12-A1-1
英文名称： Cdkn2a monoclonal antibody, clone 12-A1-1
产品编号： MAB2416
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rat monoclonal antibody raised against synthetic peptide of Cdkn2a.
- Immunogen:
- A synthetic peptide corresponding to amino acids 1-100 of mouse Cdkn2a.
- Host:
- Rat
- Reactivity:
- Mouse
- Specificity:
- This antibody is specific to the epitope residues 54-62 of murine p19ARF. This region is encoded by 1 beta of the mouse Arf gene. It does not recognize human p14Arf nor Golden hamster p13Arf.
- Form:
- Liquid
- Isotype:
- IgG2b
- Storage Buffer:
- In buffer containing 0.1% sodium azide
- Storage Instruction:
- Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:500)
Immunofluorescence (1:500)
Immunohistochemistry (Frozen sections) (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500)
Immunoprecipitation (1:500)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
Apicelli AJ, Maggi LB Jr, Hirbe AC, Miceli AP, Olanich ME, Schulte-Winkeler CL, Saporita AJ, Kuchenreuther M, Sanchez J, Weilbaecher K, Weber JD.Mol Cell Biol. 2008 Feb;28(3):1068-80. Epub 2007 Dec 10.
- 2.
- Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Klochendler-Yeivin A, Picarsky E, Yaniv M.Mol Cell Biol. 2006 Apr;26(7):2661-74.
- 3.
- Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF.Hybrid Hybridomics. 2004 Oct;23(5):293-300.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2agene (lane 1) and from wild type MEF.
- Immunofluorescence
- Immunofluorescent detection of Cdkn2a in wild type MEF at passage 5 (images 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (images 3, 4) using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416).
- Immunoprecipitation
- Immunoprecipitation of Cdkn2a monoclonal antibody, clone 12-A1-1 using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a.
- Entrez GeneID:
- 12578
- Protein Accession#:
- Q64364
- Gene Name:
- Cdkn2a
- Gene Alias:
- ARF-INK4a,Arf,INK4a-ARF,Ink4a/Arf,MTS1,Pctr1,p16,p16(INK4a),p16INK4a,p19
,p19ARF
- Gene Description:
- cyclin-dependent kinase inhibitor 2A
- Gene Ontology:
- Hyperlink
- Other Designations:
- OTTMUSP00000008143,cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4),cyclin-dependent kinase inhibitor protein,mitochondrial smARF