背景概述
肠道通透性增加和相关体重减轻是DSS结肠炎动物模型的常⻅特征。D-甘露醇是⼀种惰性小碳水化合物分⼦，可沿整个隐窝-绒毛轴吸收，⽤于评估体内肠道通透性。
图源百度
实验动物
4-5天大，约克郡小猪
实验材料
DSS（yeasen DSS 60316ES,MW36000-50000）
实验步骤
1. 以商业代乳粉配⽅饮⾷，每天喂食三次，直到实验结束；
2. 将动物分为阳性对照组（Pos），阴性对照组（Neg），实验组（Trp）;
3. 阳性对照组（Pos）：灌注DSS;阴性对照组（Neg）：灌注生理盐水;实验组（Trp）:灌注DSS+治疗药物；
4. 每只小猪每日摄入量1.25g DSS/kg，灌注5天。
实验结果
图1. 肠道通透性分析
DSS诱导致小猪体内D-甘露醇浓度升高，肠道屏障被破坏。
图2. HE病理染色切片（A为Neg组；B为Pos组）
DSS处理的Pos组隐窝结构扭曲、炎症细胞浸润到粘膜和粘膜下层、隐窝脓肿和隐窝炎。
图3. 炎症因子检测
DSS处理的Pos组中两种细胞因⼦的浓度均升高[1]。
翌圣DSS产品优势
翌圣提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：
1、高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建。
2、多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；
3、症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究。
4、简单易行，成模率高，重复性强
5、联合氧化偶氮甲烷 (azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。
建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。
产品订购
|
产品名称 |
货号 |
规格 |
价格（元） |
促销价（元） |
|
60316ES25 |
25 g |
1255.00 |
955.00 |
|
|
60316ES60 |
100 g |
3855.00 |
2655.00 |
|
|
60316ES76 |
500 g |
16855.00 |
9655.00 |
|
|
60316ES80 |
1 kg |
32855.00 |
18655.00 |
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产品已发表文章(部分)
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2022
[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143
[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451
[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076
[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869
2021
[1] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744
[2] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717
[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943
[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
2020
[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
往期精彩
1. 不同分子量葡聚糖硫酸钠选购指南：高品质DSS葡聚糖硫酸钠盐
2. 小鼠造模方法及具体步骤：Dextran Sulfate Sodium Salt（DSS）结肠炎造模解决方案 (yeasen.com)
3. DSS结肠炎造模整体解决方案：Dextran Sulfate Sodium Salt（DSS） 溃疡性结肠炎模型的建立 (yeasen.com)
参考文献
[1] Connie J. Kim, et al. Journal of Nutritional Biochemistry 21 (2010) 468–475.