胆固醇-25-羟化酶(CH25H)重组蛋白说明书

Recombinant Cholesterol-25-Hydroxylase (CH25H)   

[ PROPERTIES ]

Residues: Trp142~Asn247 (Accession # O95992),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Endoplasmic reticulum

membrane; Multi-pass membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 7.4

Predicted  Molecular Mass: 16.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ] 

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below. 

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WHLLHHKVP WLYRTFHKVHHQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT LTFHVVNIWL SVEDHSGYNFPWSTHRLVPF GWYGGVVHHD LHHSHFN

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用